Brazil ousted Venezuelan acting Gerardo Delgado.
This came days after Venezuela’s decision to expel the Brazilian ambassador to Caracas, Rui Pereira.
Venezuela says Brazil has acted illegally in the impeachment of its former left-wing president, Dilma Rousseff.
On December 23, Venezuela also expelled Canada’s temporary executors, accusing him of interfering in the interior.
On December 25, Canada announced that Venezuelan Ambassador Wilmer Barientos Fernandez, who had already been abroad, would not be allowed to return.
Venezuelan Chargé d’Affaires in Ottawa Angel Herrera was also asked to leave.
Relations with Brazil have deteriorated since President Michel Temer took office in 2016 following the dismissal of Dilma Rousseff from Congress over fiscal irregularities.
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Venezuelan President Nicolas Maduro has described her impeachment as a “right-wing coup”.
On December 23, the head of Venezuela’s powerful Constituent Assembly, Delci Rodriguez, said “diplomatic relations with Brazil will not be restored until the government restores the constitutional order it has effectively violated.”
The Brazilian government said the move showed “once again the authoritarian nature of President Maduro’s administration.”
Brazil and Canada have become outspoken critics of President Maduro.
They accuse the socialist government of Nicolas Maduro of harassing the opposition and violating human rights.
Canada imposed sanctions on senior Venezuelan officials a few months ago.
Brazil and Canada were among many countries criticizing President Maduro’s decision to convene a Constituent Assembly, which effectively replaced the opposition-controlled National Assembly.
The announcement sparked mass street protests that killed more than 120 people in four months.
Venezuela’s opposition boycotted the poll in July and also held an informal referendum, which said more than seven million Venezuelans voted against the constituent assembly.
Nicolas Maduro’s six-year term ends in 2019. He is due to run for re-election in 2018.