The Brazilian ambassador to Caracas, Rui Pereira, and the Canadian commissioner, Craig Kovalik, were expelled from Venezuela’s powerful Constituent Assembly.
The move was announced by the chairman of the Constituent Assembly, Delsi Rodriguez.
Delsey Rodriguez accused Brazil of violating the rule of law and Canada of interfering in Venezuela’s internal affairs.
Both sides have strongly criticized the move.
Ambassador Rui Pereira’s decision to expel him may have been prompted by Brazil’s recent complaint that President Nicolas Maduro was “constantly harassing the opposition.”
Canada imposed sanctions on senior Venezuelan officials a few months ago.
Venezuela’s diplomatic relations with Brazil have deteriorated after Brazilian President Michel Temer replaced left-wing leader Dilma Rousseff.
Dilma Rousseff’s impeachment has been described by Nicolas Maduro as a “right-wing coup”.
Venezuela’s main opposition parties banned from 2018 presidential election
Delsey Rodriguez told a news conference on December 23: “Diplomatic relations with Brazil will not be restored until the government restores the constitutional order it has effectively violated.”
In response, the Brazilian government said the move again showed the authoritarian nature of President Maduro’s administration.
Delsey Rodriguez accused Craig Kovalik of “constant and persistent, rude and vulgar interference in the internal affairs of Venezuela.”
Relations with Canada have been difficult for months. Earlier this year, Ottawa imposed sanctions on Venezuelan officials for alleged human rights abuses and corruption.
Last month, Foreign Minister Jorge Areaza said the sanctions were illegal and accused the Canadian government of “shameful and complete obedience to the Donald Trump administration.”
In response, Canada called for the expulsion of its caretaker, saying it would not be reduced to easing pressure on Maduro’s government.
Brazil and Canada were among many countries criticizing President Maduro’s decision to convene a Constituent Assembly, which effectively replaced the opposition-controlled National Assembly.
The announcement sparked mass street protests that killed more than 120 people in four months.
The opposition boycotted the poll in July and also held an informal referendum, which said more than seven million Venezuelans voted against the constituent assembly.
The EU and major Latin American countries have said they will not recognize the new body.
The United States, meanwhile, has imposed sanctions on President Maduro, and the Trump administration has described him as a “dictator.”
Nicolas Maduro’s six-year term ends in 2019. He is due to run for re-election next year.
The opposition accused President Maduro and his predecessor, the late Hugo Chavez, of destroying Venezuela’s economy with their socialist policies.
Venezuela has one of the highest inflation rates in the world and has been suffering from a shortage of basic commodities, including medicines, for years.